User:Carly M. Montanero/Notebook/CHEM-571/2014/01/29

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Objective

To create stock solutions for changing pH.

Procedure

  1. Shake each test tube (50, 110 ratio) of lysozyme-AuNP until fibers appear to be evenly distributed throughout.
  2. Take 1 mL aliquots from each test tube and place in 1.5 mL tubes.

Chelators

Creating Stock Solutions of Chelators


Adding Chelators to Lysozyme-AuNP Solutions

  1. Add 0.5 mL of each chelator stock solution to each 1 mL aliquot.
  2. Record effects of each chelator on each solution.

pH

Creating 1 M Buffer Solutions

Citric Acid: pH=2.99

  1. Add 42.02 g of citric acid in 180 mL distilled water.
  2. Titrate to pH 3.0 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

Phosphate: pH=6.81

  1. Add 0.0908 mol of sodium phosphate monobasic to 0.1091 mol of sodium phosphate dibasic.
  2. Fill up to 200 mL with distilled water.

Tris

  1. Dissolve 24.228 g Tris base in 180 mL distilled water.
  2. Titrate to pH 9.0 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

MES: pH=5.20

  1. Dissolve 39.04 g MES in 180 mL distilled water.
  2. Titrate to pH 5.2 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

HEPES: pH=7.05

  1. Dissolve 47.66 g HEPES in 180 mL distilled water.
  2. Titrate to pH 7 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

CHES: pH=8.97

  1. Dissolve 41.46 g CHES in 180 mL distilled water.
  2. Titrate to pH 9 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

Adding Buffers to Lysozyme-AuNP Solutions

  1. Dilute the 1 M solutions to 100 mM, 10 mM, and 1 mM.
  2. Add 1 mL of each to the lysozyme-AuNP solutions.
  3. Let sit overnight and record results.


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