User:Carly M. Montanero/Notebook/CHEM-571/2014/02/04: Difference between revisions

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==Objective==
==Objective==
*To create stock solutions of chelators.
*Synthesize lysozyme-AuNP.
*Create stock solutions of chelators.
*Add the various chelators to lysozyme-AuNP solutions.  
*Add the various chelators to lysozyme-AuNP solutions.  
*Change pH of lysozyme-AuNP solutions.
*Change pH of lysozyme-AuNP solutions.
*Record results.  
*Record results.


==Procedure==
==Procedure==

Revision as of 13:01, 4 February 2014

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Objective

  • Synthesize lysozyme-AuNP.
  • Create stock solutions of chelators.
  • Add the various chelators to lysozyme-AuNP solutions.
  • Change pH of lysozyme-AuNP solutions.
  • Record results.

Procedure

Chelators

Creating Stock Solutions of Chelators


Adding Chelators to Lysozyme-AuNP Solutions

  1. Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.
  2. Record effects of each chelator on each solution.

  • 2,2 bipyridine was used instead of 2,2 bipyridyl

pH

Adding Buffers to Lysozyme-AuNP Solutions

  1. Dilute the 1 M solutions to 100 mM, 10 mM, and 1 mM.
  2. Add 1 mL of each to the lysozyme-AuNP solutions.
  3. Let sit overnight and record results.


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