User:Carly M. Montanero/Notebook/CHEM-571/2014/02/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 14: Line 14:


==Procedure==
==Procedure==
===Synthesizing Lysozyme-AuNP===
'''Creating the Gold Stock Solution'''
# Add 0.0524 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of gold stock was 2.66 mM.
'''Creating the Lysozyme Stock Solution'''
# Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of lysozyme stock was 100.28 μM.
'''Creating Au:Protein Ratios of 40:1 and 50:1'''
# For the 40:1 ratio, add 470 μL of gold solution, 312 μL of lysozyme solution, and 4218 μL of deionized water to a test tube.
# For the 50:1 ratio, add 470 μL of gold solution, 249 μL of lysozyme solution, and 4281 μL of deionized water to a test tube.
# We made 10 tubes of each ratio.
# The samples were heated at 80°C for 4 hours.


===Chelators===
===Chelators===

Revision as of 09:58, 12 February 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Synthesize lysozyme-AuNP.
  • Create stock solutions of chelators.
  • Add the various chelators to lysozyme-AuNP solutions.
  • Change pH of lysozyme-AuNP solutions.
  • Record results.

Procedure

Synthesizing Lysozyme-AuNP

Creating the Gold Stock Solution

  1. Add 0.0524 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.66 mM.

Creating the Lysozyme Stock Solution

  1. Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 100.28 μM.

Creating Au:Protein Ratios of 40:1 and 50:1

  1. For the 40:1 ratio, add 470 μL of gold solution, 312 μL of lysozyme solution, and 4218 μL of deionized water to a test tube.
  2. For the 50:1 ratio, add 470 μL of gold solution, 249 μL of lysozyme solution, and 4281 μL of deionized water to a test tube.
  3. We made 10 tubes of each ratio.
  4. The samples were heated at 80°C for 4 hours.

Chelators

Creating Stock Solutions of Chelators


Adding Chelators to Lysozyme-AuNP Solutions

  1. Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.
  2. Record effects of each chelator on each solution.

  • 2,2 bipyridine was used instead of 2,2 bipyridyl

pH

Adding Buffers to Lysozyme-AuNP Solutions

  1. Dilute the 1 M solutions to 100 mM, 10 mM, and 1 mM.
  2. Add 1 mL of each to the lysozyme-AuNP solutions.
  3. Let sit overnight and record results.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Carly_M._Montanero/Notebook/CHEM-571/2014/02/04&oldid=771655"