User:Carly M. Montanero/Notebook/CHEM-571/2014/02/25: Difference between revisions

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==Notes==
==Notes==
[[Image:notes.2.25.jac.png|800px|]]
[[Image:notes.2.25.jac.png|700px|]]
* These results detail the samples from last Thursday with the addition of the variables to 1 mL aliquots of 30:1 ratio.
* These results detail the samples from last Thursday with the addition of the variables to 1 mL aliquots of 30:1 ratio.


Revision as of 13:10, 25 February 2014

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Objective

  • Spin down samples from Thursday and run UV-Vis.
  • Heat samples from Thursday that did not form fibers at 37°C overnight.
  • Image results of lysozyme-AuNPs synthesized with salt added before heating using UV-Vis.

Procedure

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:protein ratio were run on the AA:

Notes

  • These results detail the samples from last Thursday with the addition of the variables to 1 mL aliquots of 30:1 ratio.


  • These results detail the reaction of the different ratios synthesized with salts added before heating.


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