User:Carly M. Montanero/Notebook/CHEM-571/2014/03/04: Difference between revisions

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==Objective==
==Objective==
*  MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, 2,2 bipyridine, citric acid, MES were added to 30:1 lysozyme AuNP samples.
*  Add MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, 2,2 bipyridine, citric acid, MES to 30:1 lysozyme AuNP samples.
* Run UV-Vis of 30:1 lysozyme:AuNP (with variables added after baking) that were cooled to 4°C.  
* Run UV-Vis of 30:1 lysozyme:AuNP (with variables added after baking) that were cooled to 4°C.  


Revision as of 12:00, 26 March 2014

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Objective

  • Add MgCl2, CaCl2, NaCl, KCl, 2,2 bipyridine, citric acid, MES to 30:1 lysozyme AuNP samples.
  • Run UV-Vis of 30:1 lysozyme:AuNP (with variables added after baking) that were cooled to 4°C.

Procedure

Preparing Samples for Atomic Absorption

  • To the 30:1 lysozyme-AuNP samples synthesized with salts, 2.71 mL of the variables were added. Three concentrations for each were tested: 100 mM, 10 mM, and 1 mM.
  • For 2,2 bipyridine, the concentrations were 9 mM, 0.9 mM, and 0.09 mM.
  • The samples were left to sit at room temperature overnight.

Figures

  • Please note that the control wasn't diluted with variable like the other samples. Also the control used is the control at room temperature, not at 4°C.

Notes

Observations of 30:1 lysozyme-AuNP Samples Cooled to 4°C With Variables Added After Synthesizing


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