User:Carly M. Montanero/Notebook/CHEM-571/2014/04/01: Difference between revisions

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::* 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)
::* 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)
2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C.
2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C.


===Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature===
===Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature===
[[Image:4.1.conductivity.jac.png|800px|]]
[[Image:4.1.conductivity.jac.png|800px|]]


===Atomic Absorption Preparation===
===Atomic Absorption Preparation===

Revision as of 11:06, 1 April 2014

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Objective

  • Run UV-Vis and AA on cooled 30:1 lysozyme-AuNP samples from 3/25/14.
  • Run conductivity on room temperature 30:1 lysozyme-AuNP samples from 3/25/14.

Procedure

Adding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/14

1. Add 2.25 mL of each variable to 4.5 mL of sample.

  • The variables used were:
  • 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C.


Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature


Atomic Absorption Preparation

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)




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