User:Carly M. Montanero/Notebook/CHEM-571/2014/04/08: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Spring 2014</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Procedure== | ==Procedure== | ||
===Conductivity | ===Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature=== | ||
[[Image:4.8.conductivity.room.png|800px|]] | |||
===Atomic Absorption Preparation=== | ===Atomic Absorption Preparation=== | ||
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'''Atomic Absorption Samples''' | '''Atomic Absorption Samples''' | ||
Solutions with the following Au:lysozyme ratio at | Solutions with the following Au:lysozyme ratio at 4°C were run on the AA: | ||
* 30:1 lysozyme-AuNP with 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) | * 30:1 lysozyme-AuNP with 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) | ||
* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | * 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | ||
* 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) | * 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) | ||
==Figures== | |||
===Atomic Absorption=== | |||
[[Image:4.8.aa.all.png|700px|]] | |||
== | [[Image:4.8.2.2.bipy.png|700px|]] | ||
===UV-Vis=== | |||
*The following UV-Vis are for 30:1 lysozyme-AuNP samples made on March 26, 2014. The samples were synthesized using the method we've been using all year and variables were added added synthesis. The samples were then cooled to 4°C to see the effect on fiber formation. | |||
* Note that all for all samples with the exception of the 2,2 bipyridine samples caused almost all AuNPs to fall out of solution. | |||
[[Image:Screen_Shot_2014-04-09_at_1.29.09_PM.png]] | |||
[[Image:Screen_Shot_2014-04-09_at_1.29.34_PM.png]] | |||
[[Image:Screen_Shot_2014-04-09_at_1.29.45_PM.png]] | |||
*Note that due to the measure of accuracy of the equipment used, the percentages that are close in value might not different enough to be considered significant. This is because the values used to calculate these percentages differed by one decimal point ex: .0005 and .0004 | |||
[[Image:Screen_Shot_2014-04-09_at_1.53.20_PM.png]] | |||
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Revision as of 10:28, 15 April 2014
Biomaterials Design Lab: Spring 2014 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
ProcedureConductivity Measurements of 30:1 Lysozyme-AuNP at Room TemperatureAtomic Absorption PreparationCreating the Gold Stock Solutions
Atomic Absorption Samples Solutions with the following Au:lysozyme ratio at 4°C were run on the AA:
FiguresAtomic Absorption
UV-Vis
|