User:Carly M. Montanero/Notebook/CHEM-571/2014/04/09: Difference between revisions
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==Objective== | ==Objective== | ||
* Take conductivity measurements on | * Take conductivity measurements on 4°C solutions from 3/26/14. | ||
* Run UV-Vis and AA on solutions at | * Run UV-Vis and AA on solutions at 36°C from 3/26/14. | ||
==Procedure== | ==Procedure== | ||
===Conductivity | ===Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C=== | ||
[[Image:4.9.conductivity.4.png|800px|]] | [[Image:4.9.conductivity.4.png|800px|]] | ||
===Atomic Absorption Preparation=== | ===Atomic Absorption Preparation=== | ||
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'''Atomic Absorption Samples''' | '''Atomic Absorption Samples''' | ||
Solutions with the following Au:lysozyme ratio at | Solutions with the following Au:lysozyme ratio at 36°C were run on the AA: | ||
* 30:1 lysozyme-AuNP with 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) | * 30:1 lysozyme-AuNP with 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) | ||
* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | * 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | ||
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==Figures== | ==Figures== | ||
===Atomic Absorption=== | |||
[[Image:4.9.aa.hot.png|700px|]] | [[Image:4.9.aa.hot.png|700px|]] | ||
[[Image:4.9.aa.hot.2.2.bipy.png|700px|]] | [[Image:4.9.aa.hot.2.2.bipy.png|700px|]] | ||
==UV-Vis Images== | |||
*Please note that very few samples actually had suspended nanoparticles in solution. | |||
[[Image:Screen_Shot_2014-04-15_at_7.37.48_PM.png]] | |||
[[Image:Screen_Shot_2014-04-15_at_7.39.02_PM.png]] | |||
[[Image:Screen_Shot_2014-04-15_at_7.39.52_PM.png]] | |||
[[Image:Screen_Shot_2014-04-16_at_3.04.23_PM.png]] | |||
*Note that highlighted samples are the only samples with enough of a recognizable peak to calculate an equilibrium constant. Some 2,2 bipyridine data is being left out because it showed no variation from the control. | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 13:49, 21 April 2014
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Objective
ProcedureConductivity Measurements of 30:1 Lysozyme-AuNP at 4°C
Atomic Absorption PreparationCreating the Gold Stock Solutions
Atomic Absorption Samples Solutions with the following Au:lysozyme ratio at 36°C were run on the AA:
FiguresAtomic Absorption
UV-Vis Images
|