User:Carly M. Montanero/Notebook/CHEM-571/2014/04/09: Difference between revisions

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==Procedure==
==Procedure==


===Conductivity Measurement of Pure Variables at 4°C Temperature===
===Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C===


[[Image:4.9.conductivity.4.png|800px|]]
[[Image:4.9.conductivity.4.png|800px|]]


===Atomic Absorption Preparation===
===Atomic Absorption Preparation===
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==Figures==
==Figures==
===Atomic Absorption===
[[Image:4.9.aa.hot.png|700px|]]
[[Image:4.9.aa.hot.png|700px|]]


[[Image:4.9.aa.hot.2.2.bipy.png|700px|]]
[[Image:4.9.aa.hot.2.2.bipy.png|700px|]]


==UV-Vis Images==
*Please note that very few samples actually had suspended nanoparticles in solution.
[[Image:Screen_Shot_2014-04-15_at_7.37.48_PM.png]]
[[Image:Screen_Shot_2014-04-15_at_7.39.02_PM.png]]
[[Image:Screen_Shot_2014-04-15_at_7.39.52_PM.png]]
[[Image:Screen_Shot_2014-04-16_at_3.04.23_PM.png]]
*Note that highlighted samples are the only samples with enough of a recognizable peak to calculate an equilibrium constant. Some 2,2 bipyridine data is being left out because it showed no variation from the control.
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__NOTOC__
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Revision as of 13:49, 21 April 2014

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Objective

  • Take conductivity measurements on 4°C solutions from 3/26/14.
  • Run UV-Vis and AA on solutions at 36°C from 3/26/14.

Procedure

Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C


Atomic Absorption Preparation

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at 36°C were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

Figures

Atomic Absorption


UV-Vis Images

  • Please note that very few samples actually had suspended nanoparticles in solution.

  • Note that highlighted samples are the only samples with enough of a recognizable peak to calculate an equilibrium constant. Some 2,2 bipyridine data is being left out because it showed no variation from the control.


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