Objective
- Take conductivity measurements on 4°C solutions from 3/26/14.
- Run UV-Vis and AA on solutions at 36°C from 3/26/14.
Procedure
Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C
Atomic Absorption Preparation
Creating the Gold Stock Solutions
- Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
- Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
- Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
- Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
- Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.
Atomic Absorption Samples
Solutions with the following Au:lysozyme ratio at 36°C were run on the AA:
- 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
- 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
- 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)
Figures
Atomic Absorption
UV-Vis Images
- Please note that very few samples actually had suspended nanoparticles in solution.
- Note that highlighted samples are the only samples with enough of a recognizable peak to calculate an equilibrium constant. Some 2,2 bipyridine data is being left out because it showed no variation from the control.
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