User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/11/16: Difference between revisions

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#The emission and exit slit were  
#The emission and exit slit were  


==Data==
AuNP pH10 scans
[[Image:Fluorescence pH 10 scans.png|600px]]
Wavelength of Myoglobin AuNP's at pH 10
[[Image:Fluorescence pH 10 wavelength.png|600px]]
Integrated intensity of Myoglobin AuNP's at pH 10
[[Image:Fluorescence pH 10 integrated intensity.png|600


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Revision as of 10:22, 6 December 2016

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Flourescence

Procedure

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 2.96mM
      1. (0.25mM)(3.0mL)=(XmL)(2.96mM)
      2. XmL= .253mL= 253uL Au
    2. Myoglobin 141.2uM
      1. (12.5uM)(3.0mL)=(XmL)(141.2uM)
      2. X=0.265mL= 265uL Myoglobin
    3. 300uL 1mM HCl
    4. Water
      1. Volume of water= 3000uL- 253uL-265uL-300uL= 2182uL
  2. Absorbance measurements were taken and saved every 3 minutes for 3 hours
  3. The instrument was set at 80 degrees Celsius
  4. The scan range parameters were start at 310nm and ending at 540nm
  5. The emission and exit slit were

Data

AuNP pH10 scans

Wavelength of Myoglobin AuNP's at pH 10

Integrated intensity of Myoglobin AuNP's at pH 10

[[Image:Fluorescence pH 10 integrated intensity.png|600


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