User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/11/30: Difference between revisions
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(Autocreate 2016/11/30 Entry for User:Cassidy_B._Hart/Notebook/CHEM-471:_Gold_&_Silver_Nanoparticles) |
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== | ==Ocean Optics== | ||
==Procedure== | |||
First pH 11 was made: | |||
#Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total | |||
##Au 3.37mM | |||
###(0.25mM)(3.0mL)=(XmL)(3.37mM) | |||
###XmL= .223mL= 223uL Au | |||
##Myoglobin 230uM | |||
###(12.5uM)(3.0mL)=(XmL)(230uM) | |||
###X=0.163mL= 163uL Myoglobin | |||
##3uL 1M NaOH | |||
##Water | |||
###Volume of water= 3000uL- 223uL-163uL-3uL= 2611uL | |||
##Scans were taken at 80oC every 2 minutes for 3 hours | |||
Second pH 12 was made: | |||
#Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total | |||
##Au 3.37mM | |||
###(0.25mM)(3.0mL)=(XmL)(3.37mM) | |||
###XmL= .223mL= 223uL Au | |||
##Myoglobin 230uM | |||
###(12.5uM)(3.0mL)=(XmL)(230uM) | |||
###X=0.163mL= 163uL Myoglobin | |||
##30uL 1M NaOH | |||
##Water | |||
###Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL | |||
==Data== | |||
pH 11 Data | |||
Absorbance | |||
[[Image:PH11 absorbance ocean optics.png|600px]] | |||
Wavelength | |||
[[Image:PH 11 wavelength ocean optics.png|600px]] | |||
pH 12 Data | |||
Absorbance | |||
[[Image:PH 12 absorbance ocean optics.png|600px]] | |||
Wavelength | |||
[[Image:PH 12 wavelength ocean optics.png|600px]] | |||
==Notes== | |||
No NPs formed. The graphs of the wavelength are just the wavelength bouncing around the minimum value and the graphs of absorbance show minute increases/decreases in absorbance. | |||
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Revision as of 11:51, 6 December 2016
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Ocean OpticsProcedureFirst pH 11 was made:
Second pH 12 was made:
DatapH 11 Data Absorbance Wavelength
Absorbance Wavelength NotesNo NPs formed. The graphs of the wavelength are just the wavelength bouncing around the minimum value and the graphs of absorbance show minute increases/decreases in absorbance. |