User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/11/30

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Ocean Optics

Procedure

First pH 11 was made:

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.37mM
      1. (0.25mM)(3.0mL)=(XmL)(3.37mM)
      2. XmL= .223mL= 223uL Au
    2. Myoglobin 230uM
      1. (12.5uM)(3.0mL)=(XmL)(230uM)
      2. X=0.163mL= 163uL Myoglobin
    3. 3uL 1M NaOH
    4. Water
      1. Volume of water= 3000uL- 223uL-163uL-3uL= 2611uL
    5. Scans were taken at 80oC every 2 minutes for 3 hours

Second pH 12 was made:

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.37mM
      1. (0.25mM)(3.0mL)=(XmL)(3.37mM)
      2. XmL= .223mL= 223uL Au
    2. Myoglobin 230uM
      1. (12.5uM)(3.0mL)=(XmL)(230uM)
      2. X=0.163mL= 163uL Myoglobin
    3. 30uL 1M NaOH
    4. Water
      1. Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL


Data

pH 11 Data

Absorbance

Wavelength


pH 12 Data

Absorbance

Wavelength

Notes

No NPs formed. The graphs of the wavelength are just the wavelength bouncing around the minimum value and the graphs of absorbance show minute increases/decreases in absorbance.


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