User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/09/07: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 29: Line 29:
*removed samples at 12:10. Oven temp 74C. Took UV spectra. Replaced samples in oven at 12:20, oven temp 72C
*removed samples at 12:10. Oven temp 74C. Took UV spectra. Replaced samples in oven at 12:20, oven temp 72C
*removed samples at 12:50. Oven temp 74C. Took UV spectra. Replace samples 1:05, oven temp 72C.
*removed samples at 12:50. Oven temp 74C. Took UV spectra. Replace samples 1:05, oven temp 72C.
*removed samples at 1:35. Oven temp 65C. Took UV spectra.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

Revision as of 10:46, 7 September 2011

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Objective

Repeat Experiment 1 from August 31. Synthesize Gold nanoparticles and take a UV spectra.

Description

  1. Combine 1 mL of 15 μM BSA and 1 mL of 2.5mM HAuCl4 in two sample tubes.
  2. Fill one tube with 8 mL of 50 mM Acetate Buffer pH 3.6
  3. Fill the second test tube with 8 mL of water.
  4. Place in over at 80C.
  5. Take UV spectra every 30 min.

Data

  • Add data and results here...

Notes

  • FInal concentration 0.25mM HAuCl4 and 1.5μM BSA in each tube.
  • Spectra taken of water and buffer samples at t=0. Placed in oven at 11:40 at 79C.
  • Spectra taken of blank water and buffer samples.
  • removed samples at 12:10. Oven temp 74C. Took UV spectra. Replaced samples in oven at 12:20, oven temp 72C
  • removed samples at 12:50. Oven temp 74C. Took UV spectra. Replace samples 1:05, oven temp 72C.
  • removed samples at 1:35. Oven temp 65C. Took UV spectra.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Catherine_Koenigsknecht/Notebook/Experimental_Biological_Chemistry/2011/09/07&oldid=534938"