User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/09/20: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 14: Line 14:


==Description==
==Description==
#  
# previously expressed cells were thawed in water til liquid then sonicated for 1 min 30 sec in 15 sec bursts. Between the 15 secs the protein content was measured using a bradford assay.
# The tubes were tehn centrifuged at 9000 rpm for 30 minutes.
#


==Data==
==Data==

Revision as of 16:46, 20 September 2011

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Objective

Extracting protein from the cell cultures.

Description

  1. previously expressed cells were thawed in water til liquid then sonicated for 1 min 30 sec in 15 sec bursts. Between the 15 secs the protein content was measured using a bradford assay.
  2. The tubes were tehn centrifuged at 9000 rpm for 30 minutes.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Catherine_Koenigsknecht/Notebook/Experimental_Biological_Chemistry/2011/09/20&oldid=539301"