User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/10/05

From OpenWetWare
Jump to navigationJump to search
Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Objective

Take fluorescence spectra of the maltose-binding protein (MBP).

Description

The calculated concentration of the MBP protein on 9/13/11 was 10.714μM

  1. First, the 10.714μM MBP was diluted by mixing 1397μL of it with 1603μL of 50mM Tris buffer, pH 7.55. This gave a final concentration of 4.989μM.
    • The intensities of this was taken from 310nm-500nm when excited at 290nm.
  2. Then, 300μL of the 4.989μM MBP was diluted with 2700μL of the same Tris buffer for a final concentration of 0.4989μM.
    • The intensities of this was taken from 310nm-500nm when excited at 290nm.
  3. Then, 300μL of the 0.4989μM MBP was diluted with 2700μL of the same Tris buffer for a final concentration of 49.89nM.
    • The intensities of this was taken from 310nm-500nm when excited at 290nm.
  4. Then, 300μL of the 49.89nM MBP was diluted with 2700μL of the same Tris buffer for a final concentration of 4.989nM.
    • The intensities of this was taken from 310nm-500nm when excited at 290nm.
  5. Then, 300μL of the 4.989nM MBP was diluted with 2700μL of the same Tris buffer for a final concentration of 0.4989nM.
    • The intensities of this was taken from 310nm-500nm when excited at 290nm.

Data

  • The intensities of the different concentrations of MBP were corrected with the intensities of the Tris buffer:


The intensities divided by the concentrations of MBP vs. the wavelengths:

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Catherine_Koenigsknecht/Notebook/Experimental_Biological_Chemistry/2011/10/05&oldid=573826"