User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/10/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 23: Line 23:


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
#Abs at 490nm = 1.144AU for 20x dilute
*A=bcε, b=1cm, ε=42000M-1cm-1
*c=27.24μM for 20x dilute
#actual concentration is 544.76μM
*concentration was supposed to be 277.231μM (.005g/(515.3g/mol)/(.035L)*106)
#Concentration of cysteine = ((118g/L)*(1.96*10-5L))/(121.16 g/mol)/(.035L)*106 = 545.395μM
 




Revision as of 09:41, 26 December 2011

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Objective

Make a labeled cysteine fluorophore.

Description

  1. Measure out 5mg of 5-(Iodoacetamido)fluorescein and put in a 50mL Falcon tube.
  2. Add 19.6μL of 118mg/mL cysteine to the 50mL Falcon tube.
  3. Add 35mL of 50mM Tris, pH 7.5.
  4. Add 100μL of 2.25M DTT after about two hours of the reaction occurring at room temperature.

Data

  • Add data and results here...

Notes

  1. Abs at 490nm = 1.144AU for 20x dilute
  • A=bcε, b=1cm, ε=42000M-1cm-1
  • c=27.24μM for 20x dilute
  1. actual concentration is 544.76μM
  • concentration was supposed to be 277.231μM (.005g/(515.3g/mol)/(.035L)*106)
  1. Concentration of cysteine = ((118g/L)*(1.96*10-5L))/(121.16 g/mol)/(.035L)*106 = 545.395μM


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Catherine_Koenigsknecht/Notebook/Experimental_Biological_Chemistry/2011/10/18&oldid=573838"