User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2012/02/14: Difference between revisions

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==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
*Extracting Protein from cell cultures


==Description==
==Description==
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#The cells were sonicated for 15 seconds at a time with approximately one minute on ice between sonications. In total the cells were sonicated for 90 seconds.
#The cells were sonicated for 15 seconds at a time with approximately one minute on ice between sonications. In total the cells were sonicated for 90 seconds.
#The cells were centrifuged at 9000g for 30 minutes at 4°C.
#The cells were centrifuged at 9000g for 30 minutes at 4°C.
#Purify supernatant using syringe filter
#Store purified supernatant in refrigerator


Perform Bradford Assay
Perform Bradford Assay

Revision as of 12:50, 14 February 2012

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Entry title

Objective

  • Extracting Protein from cell cultures

Description

Extraction

  1. Defrost cells that were stored at -20°C.
  2. The cells were sonicated for 15 seconds at a time with approximately one minute on ice between sonications. In total the cells were sonicated for 90 seconds.
  3. The cells were centrifuged at 9000g for 30 minutes at 4°C.
  4. Purify supernatant using syringe filter
  5. Store purified supernatant in refrigerator

Perform Bradford Assay

  1. test each fraction using Bio-Rad Protein Assay using Bradford "by-eye"

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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