User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23

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==Entry title==
 
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Characterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol
 
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==Objective==
 
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1) FInish Proposal for experiment: https://docs.google.com/folder/d/0B_01BNMu9uSudFZCSnVncjkxQXM/edit?docId=1M2Xy9AKW7BVladIhxcBYcx8lwDc9rqNH4ISIj0xfWy8
 
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2) Outline Protocol for next class
 
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==Description==
 
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*Calculation: Dilution required to yield 10mL Adenosine buffer
 
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*Km for Adenosine = 23 uM
 
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*Phosphate buffer required: 300mL
 
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==Data==
 
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* Conversions and Measurements: Solution
 
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*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2013/01/29| next Tuesday]].
 
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[[Image:ADA_kinetics_table.png]]
 
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* Procedure: Buffer https://docs.google.com/spreadsheet/ccc?key=0AvpBsSn1622LdE9rUThCVkplbmxDbnFPRHh2NFJwcXc#gid=2
 
==Notes==
==Notes==

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