User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23

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==Entry title==
 
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Characterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol
 
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==Objective==
 
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1) FInish Proposal for experiment: https://docs.google.com/folder/d/0B_01BNMu9uSudFZCSnVncjkxQXM/edit?docId=1M2Xy9AKW7BVladIhxcBYcx8lwDc9rqNH4ISIj0xfWy8
 
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2) Outline Protocol for next class
 
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==Description==
 
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*Calculation: Dilution required to yield 10mL Adenosine buffer
 
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*Km for Adenosine = 23 uM
 
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*Phosphate buffer required: 300mL
 
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==Data==
 
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* Conversions and Measurements: Solution
 
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*The following table outlines the final concentrations and volumes of the solutions used in the ADA kinetics assay to be performed [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2013/01/29]].
 
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[[Image:ADA_kinetics_table.png]]
 
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* Procedure: Buffer
 
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#Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
 
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#Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
 
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#Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
 
==Notes==
==Notes==

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