# User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23

(Difference between revisions)
 Revision as of 16:22, 23 January 2013 (view source) (→Data)← Previous diff Current revision (17:44, 8 May 2013) (view source) (→Data) (3 intermediate revisions not shown.) Line 6: Line 6: | colspan="2"| | colspan="2"| - ==Entry title== - Characterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol - ==Objective== - 1) FInish Proposal for experiment: https://docs.google.com/folder/d/0B_01BNMu9uSudFZCSnVncjkxQXM/edit?docId=1M2Xy9AKW7BVladIhxcBYcx8lwDc9rqNH4ISIj0xfWy8 - 2) Outline Protocol for next class - ==Data== - * Conversions and Measurements: Solution - - *The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed - - [[Image:ADA_kinetics_table.png|px80]] - - *To prepare a 30 mM stock solution of adenosine: - - $\frac{0.030mol}{L}$ of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172g}{L}$ of adenosine in buffer - - * Procedure: Buffer - #Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4) - #Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C. - #Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O) ==Notes== ==Notes==

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