User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23

Entry title

Characterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol

Objective

1) FInish Proposal for experiment: https://docs.google.com/folder/d/0B_01BNMu9uSudFZCSnVncjkxQXM/edit?docId=1M2Xy9AKW7BVladIhxcBYcx8lwDc9rqNH4ISIj0xfWy8

2) Outline Protocol for next class

Description

• Calculation: Dilution required to yield 10mL Adenosine buffer

• Km for Adenosine = 23 uM

• Phosphate buffer required: 300mL

Data

• Conversions and Measurements: Solution

• The following table outlines the final concentrations and volumes of the solutions used in the ADA kinetics assay to be performed

• Procedure: Buffer

1. Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
2. Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
3. Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Notes

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