User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23: Difference between revisions
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2) Outline Protocol for next class | 2) Outline Protocol for next class | ||
* | ==Data== | ||
* Conversions and Measurements: Solution | |||
*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed | |||
[[Image:ADA_kinetics_table.png|px80]] | |||
*To prepare a 30 mM stock solution of adenosine: | |||
<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24 g}{1 mol}</math> = <math>\frac{8.0172g}{L}</math> of adenosine in buffer | |||
*To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer | |||
* Procedure: Buffer | * Procedure: Buffer | ||
#Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4) | |||
#Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C. | |||
#Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O) | |||
==Notes== | ==Notes== |
Revision as of 13:25, 23 January 2013
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Entry titleCharacterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol Objective1) FInish Proposal for experiment: https://docs.google.com/folder/d/0B_01BNMu9uSudFZCSnVncjkxQXM/edit?docId=1M2Xy9AKW7BVladIhxcBYcx8lwDc9rqNH4ISIj0xfWy8 2) Outline Protocol for next class
Data
[math]\displaystyle{ \frac{0.030mol}{L} }[/math] of adenosine × [math]\displaystyle{ \frac{267.24 g}{1 mol} }[/math] = [math]\displaystyle{ \frac{8.0172g}{L} }[/math] of adenosine in buffer
NotesThis area is for any observations or conclusions that you would like to note.
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