User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23

From OpenWetWare

Jump to: navigation, search
Biomaterials Design Lab Main project page
Previous entry      Next entry

Entry title

Characterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol

Objective

1) FInish Proposal for experiment: https://docs.google.com/folder/d/0B_01BNMu9uSudFZCSnVncjkxQXM/edit?docId=1M2Xy9AKW7BVladIhxcBYcx8lwDc9rqNH4ISIj0xfWy8

2) Outline Protocol for next class

Description

  • Calculation: Dilution required to yield 10mL Adenosine buffer
  • Km for Adenosine = 23 uM
  • Phosphate buffer required: 300mL

Data

  • Conversions and Measurements: Solution
  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

Image:ADA_kinetics_table.png

  • Procedure: Buffer
  1. Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
  2. Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
  3. Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Personal tools