User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23: Difference between revisions

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==Entry title==
Characterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol


==Objective==
1) FInish Proposal for experiment: https://docs.google.com/folder/d/0B_01BNMu9uSudFZCSnVncjkxQXM/edit?docId=1M2Xy9AKW7BVladIhxcBYcx8lwDc9rqNH4ISIj0xfWy8


2) Outline Protocol for next class


==Description==
*Calculation: Dilution required to yield 10mL Adenosine buffer


*Km for Adenosine = 23 uM


*Phosphate buffer required: 300mL
==Data==
* Conversions and Measurements: Solution
*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2013/01/29| next Tuesday]].
[[Image:ADA_kinetics_table.png]]
* Procedure: Buffer
#Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
#Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
#Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)


==Notes==
==Notes==

Revision as of 14:44, 8 May 2013

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Notes

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