User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/30: Difference between revisions

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*pH was brought to 7.4 using NaOH
*pH was brought to 7.4 using NaOH


==Notes==
==Preparation of ADA aliquots==
This area is for any observations or conclusions that you would like to note.
*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] measured out 1.7g 0.5unit/mL ADA
 
*Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

Revision as of 16:53, 31 January 2013

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Entry title

Solution Preparation prepared by Michael Nagle and me: Lab entry written by Michael

Objective

  • Prepare stock solutions of adenosine and phosphate buffer
  • Prepare aliquots of ADA stock solution
    • Stocks will be used for ADA activity assay next week

Description

  • 10mL of 15mM solution of adenosine was prepared
    • .015M = x/.01
    • 0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
    • 401mg adenosine was weighed and added to 10mL phosphate buffer.
  • The 15mM solution was diluted to 5mM
    • .01L * .015M = .005M * x
    • 0.03L total volume for 5mM
    • 0.02L buffer was added to bring the solution to 30mL and 5mM

Preparation of phosphate buffer

  • 3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
  • pH was brought to 7.4 using NaOH

Preparation of ADA aliquots

  • Dhea Patel measured out 1.7g 0.5unit/mL ADA
  • Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
  • This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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