User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/06

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(Description)
Current revision (16:49, 8 May 2013) (view source)
(Notes)
 
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Learn how to maintain an OpenWetWare Notebook.
Learn how to maintain an OpenWetWare Notebook.
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==Description==
 
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#UVProbe was opened.
 
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##Window > 1. Kinetics
 
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##Methods Icon
 
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###Wavelength: 265nm
 
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###Duration: 300 seconds (5minutes)
 
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###OK
 
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#Shimadzu CPS-Controller was set to 25°C.
 
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##wait for the temperature to raise to 25°C
 
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#place the sample in the cell and click start.
 
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*Phosphate buffer was used as a blank and was used to create the baseline.
 
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*Kinetics was run at 265nm using 2.38mL phosphate buffer, 600μL of 500μM stock adenosine, and 20μL ADA (as outlined in the table below).
 
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**The velocity was determined by clicking Operations > Activity Table > dAbs. The average of the dAbs values is the average slope (velocity).
 
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*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
 
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**150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6uL new stock for ADA assay
 
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**200 uM
 
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**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 
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***[[Image:Screen_Shot_2013-02-05_at_9.01.20_PM.png]]
 
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**This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 
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***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]]
 
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==Data==
 
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* Add data and results here...
 
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==Notes==
 
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This area is for any observations or conclusions that you would like to note.
 

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