User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/06

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Current revision (17:49, 8 May 2013) (view source)
(Notes)
 
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==Data==
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Average Velocity over 30s (nm/sec)'''
 
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|-
 
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| 200||0.00021
 
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|-
 
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| 100||0.000183333
 
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|-
 
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| 40||0.00012
 
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|-
 
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| 16||0.0001
 
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|-
 
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| 6.4||0.000047
 
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|-
 
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| 2.56||0.000033
 
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|}
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''1/[adenosine] (1/uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''1/[velocity] (sec/nm)'''
 
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|-
 
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| 0.005||4761.904762
 
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|-
 
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| 0.01||5454.545455
 
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|-
 
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| 0.025||8333.333333
 
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|-
 
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| 0.0625||10000
 
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|-
 
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| 0.15625||21276.59574
 
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|-
 
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| 0.390625||30303.0303
 
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|}
 
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*Lineweaver Burke Plot:
 
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[[Image:ConcentrationvsVelocity-1.png]]
 
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*Concentration Adenosine vs Velocity:
 
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[[Image:LineweaverBurkePlot-1.png]]
 
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{| {{table}}
 
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| -1/Vmax||6163.1
 
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|-
 
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| '''Vmax'''||'''0.000162256 nm/sec'''
 
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|-
 
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| -1/Km||-0.093
 
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|-
 
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| '''Km'''||'''10.75 uM'''
 
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|}
 
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==Notes==
 
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Concentration recalculations were taken from Dhea Patel
 
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Deleted concentration 2.56 uM point from graphs
 
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