User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/06: Difference between revisions
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==Description== | ==Description== | ||
*5mM adenosine stock solution was | #UVProbe was opened. | ||
##Window > 1. Kinetics | |||
##Methods Icon | |||
###Wavelength: 265nm | |||
###Duration: 300 seconds (5minutes) | |||
###OK | |||
#Shimadzu CPS-Controller was set to 25°C. | |||
##wait for the temperature to raise to 25°C | |||
#place the sample in the cell and click start. | |||
*Phosphate buffer was used as a blank and was used to create the baseline. | |||
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted: | |||
**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions. | **This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions. | ||
***[[Image:Screen_Shot_2013-02-05_at_9.01.20_PM.png]] | ***[[Image:Screen_Shot_2013-02-05_at_9.01.20_PM.png]] |
Revision as of 09:53, 6 February 2013
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