User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/06

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Objective

Learn how to maintain an OpenWetWare Notebook.


Data

Concentration of Adenosine (uM) Average Velocity over 30s (nm/sec)
2000.00021
1000.000183333
400.00012
160.0001
6.40.000047
2.560.000033


1/[adenosine] (1/uM) 1/[velocity] (sec/nm)
0.0054761.904762
0.015454.545455
0.0258333.333333
0.062510000
0.1562521276.59574
0.39062530303.0303


  • Lineweaver Burke Plot:

Image:ConcentrationvsVelocity-1.png

  • Concentration Adenosine vs Velocity:

Image:LineweaverBurkePlot-1.png

-1/Vmax6163.1
Vmax0.000162256 nm/sec
-1/Km-0.093
Km10.75 uM

Notes

Concentration recalculations were taken from Dhea Patel Deleted concentration 2.56 uM point from graphs

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