User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(→Concentration Tables)
(→Concentration Tables)
Line 28: Line 28:
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
|-
|-
-
| 5000||200||800||1000||3
+
| 5000||200||800||1000||1
|-
|-
-
| 5000||150||850||750||3
+
| 5000||150||850||750||1
|-
|-
-
| 5000||100||900||500||3
+
| 5000||100||900||500||1
|-
|-
-
| 5000||40||960||200||3
+
| 5000||40||960||200||1
|-
|-
-
| 5000||16||984||80||3
+
| 5000||16||984||80||1
|-
|-
-
| 5000||6.4||993.6||32||3
+
| 5000||6.4||993.6||32||1
 +
|}
 +
 
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
 +
|-
 +
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||750||0.6||150||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||500||0.6||100||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||200||0.6||40||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||80||0.6||16||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
|}
|}

Revision as of 13:30, 15 February 2013

Biomaterials Design Lab Main project page
Previous entry      Next entry

Entry title

Objective

Learn how to maintain an OpenWetWare Notebook.

Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
    • Dilution Tables are located below

Concentration Tables

This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.

[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
500020080010001
50001508507501
50001009005001
5000409602001
500016984801
50006.4993.6321


[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (mL) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Total Volume (mL)
0.50.020.0033310000.62002.383
0.50.020.003337500.61502.383
0.50.020.003335000.61002.383
0.50.020.003332000.6402.383
0.50.020.00333800.6162.383
0.50.020.00333320.66.42.383

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "http://www.openwetware.org/wiki/User:Catherine_Koenigsknecht/Notebook/Experimental_Biological_Chemistry/2013/02/12"
Personal tools