User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12

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(Objective)
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==Objective==
==Objective==
-
Learn how to maintain an OpenWetWare Notebook.
+
Repeat ADA Assay 2 times for additional data
==Description==
==Description==
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
 +
**Dilution Tables are located below
==Concentration Tables==
==Concentration Tables==
 +
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 +
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
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| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
|-
|-
-
| 5000||200||800||1000||3
+
| 5000||200||800||1000||1
 +
|-
 +
| 5000||150||850||750||1
 +
|-
 +
| 5000||100||900||500||1
 +
|-
 +
| 5000||40||960||200||1
 +
|-
 +
| 5000||16||984||80||1
 +
|-
 +
| 5000||6.4||993.6||32||1
 +
|}
 +
 
 +
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
 +
|-
 +
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||750||0.6||150||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||500||0.6||100||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||200||0.6||40||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||80||0.6||16||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
 +
|}
 +
 
 +
==Data==
 +
Run#1
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
 +
|-
 +
| 200||0.000116022
 +
|-
 +
| 150||0.000192818
 +
|-
 +
| 100||0.000139227
 +
|-
 +
| 40||5.55556E-06
 +
|-
 +
| 16||6.68508E-05
 +
|-
 +
| 6.4||0.000039
 +
|}
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
 +
|-
 +
| 0.005||8619.047619
 +
|-
 +
| 0.006666667||5186.246418
 +
|-
 +
| 0.01||7182.539683
 +
|-
 +
| 0.025||180000
 +
|-
 +
| 0.0625||14958.67769
 +
|-
 +
| 0.15625||25641.02564
 +
|}
 +
 
 +
 
 +
Run#2
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
 +
|-
 +
| 200||0.00021989
 +
|-
 +
| 150||0.000147514
 +
|-
 +
| 100||0.000144751
 +
|-
 +
| 40||0.00010884
 +
|-
 +
| 16||8.12155E-05
 +
|-
 +
| 6.4||3.8674E-05
 +
|}
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
 +
|-
 +
| 0.005||4547.738693
|-
|-
-
| 5000||150||850||750||3
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| 0.006666667||6779.026217
|-
|-
-
| 5000||100||900||500||3
+
| 0.01||6908.396947
|-
|-
-
| 5000||40||960||200||3
+
| 0.025||9187.817259
|-
|-
-
| 5000||16||984||80||3
+
| 0.0625||12312.92517
|-
|-
-
| 5000||6.4||993.6||32||3
+
| 0.15625||25857.14286
|}
|}

Revision as of 14:14, 15 February 2013

Biomaterials Design Lab Main project page
Previous entry      Next entry

Entry title

Objective

Repeat ADA Assay 2 times for additional data

Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
    • Dilution Tables are located below

Concentration Tables

This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.

[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
500020080010001
50001508507501
50001009005001
5000409602001
500016984801
50006.4993.6321

This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.

[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (mL) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Total Volume (mL)
0.50.020.0033310000.62002.383
0.50.020.003337500.61502.383
0.50.020.003335000.61002.383
0.50.020.003332000.6402.383
0.50.020.00333800.6162.383
0.50.020.00333320.66.42.383

Data

Run#1

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
2000.000116022
1500.000192818
1000.000139227
405.55556E-06
166.68508E-05
6.40.000039
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.0058619.047619
0.0066666675186.246418
0.017182.539683
0.025180000
0.062514958.67769
0.1562525641.02564


Run#2

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
2000.00021989
1500.000147514
1000.000144751
400.00010884
168.12155E-05
6.43.8674E-05
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.0054547.738693
0.0066666676779.026217
0.016908.396947
0.0259187.817259
0.062512312.92517
0.1562525857.14286

Notes

This area is for any observations or conclusions that you would like to note.


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