User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12: Difference between revisions
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==Objective== | ==Objective== | ||
Repeat ADA Assay 2 times for additional data | |||
==Description== | ==Description== | ||
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| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)''' | | align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)''' | ||
|- | |- | ||
| 5000||200||800||1000|| | | 5000||200||800||1000||1 | ||
|- | |- | ||
| 5000||150||850||750|| | | 5000||150||850||750||1 | ||
|- | |- | ||
| 5000||100||900||500|| | | 5000||100||900||500||1 | ||
|- | |- | ||
| 5000||40||960||200|| | | 5000||40||960||200||1 | ||
|- | |- | ||
| 5000||16||984||80|| | | 5000||16||984||80||1 | ||
|- | |- | ||
| 5000||6.4||993.6||32||3 | | 5000||6.4||993.6||32||1 | ||
|} | |||
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)''' | |||
|- | |||
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||750||0.6||150||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||500||0.6||100||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||200||0.6||40||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||80||0.6||16||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3 | |||
|} | |||
==Data== | |||
Run#1 | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)''' | |||
|- | |||
| 200||0.000116022 | |||
|- | |||
| 150||0.000192818 | |||
|- | |||
| 100||0.000139227 | |||
|- | |||
| 40||5.55556E-06 | |||
|- | |||
| 16||6.68508E-05 | |||
|- | |||
| 6.4||0.000039 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)''' | |||
|- | |||
| 0.005||8619.047619 | |||
|- | |||
| 0.006666667||5186.246418 | |||
|- | |||
| 0.01||7182.539683 | |||
|- | |||
| 0.025||180000 | |||
|- | |||
| 0.0625||14958.67769 | |||
|- | |||
| 0.15625||25641.02564 | |||
|} | |||
Run#2 | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)''' | |||
|- | |||
| 200||0.00021989 | |||
|- | |||
| 150||0.000147514 | |||
|- | |||
| 100||0.000144751 | |||
|- | |||
| 40||0.00010884 | |||
|- | |||
| 16||8.12155E-05 | |||
|- | |||
| 6.4||3.8674E-05 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)''' | |||
|- | |||
| 0.005||4547.738693 | |||
|- | |||
| 0.006666667||6779.026217 | |||
|- | |||
| 0.01||6908.396947 | |||
|- | |||
| 0.025||9187.817259 | |||
|- | |||
| 0.0625||12312.92517 | |||
|- | |||
| 0.15625||25857.14286 | |||
|} | |} | ||
Revision as of 11:14, 15 February 2013
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Entry titleObjectiveRepeat ADA Assay 2 times for additional data Description
Concentration TablesThis table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
DataRun#1
NotesThis area is for any observations or conclusions that you would like to note.
|