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| |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> |
| |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} |
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| ==Objective==
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| Learn how to maintain an OpenWetWare Notebook.
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| ==Description==
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| *Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
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| *5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
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| **Dilution Tables are located below
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| ==Concentration Tables==
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| This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
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| | align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
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| |-
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| | 5000||200||800||1000||1
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| |-
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| | 5000||150||850||750||1
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| | 5000||100||900||500||1
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| | 5000||40||960||200||1
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| |-
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| | 5000||16||984||80||1
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| |-
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| | 5000||6.4||993.6||32||1
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| |}
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| This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
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| | align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
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| | align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
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| | align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
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| | align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
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| |-
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| | 0.5||0.02||0.00333||1000||0.6||200||2.38||3
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| |-
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| | 0.5||0.02||0.00333||750||0.6||150||2.38||3
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| | 0.5||0.02||0.00333||500||0.6||100||2.38||3
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| | 0.5||0.02||0.00333||200||0.6||40||2.38||3
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| | 0.5||0.02||0.00333||80||0.6||16||2.38||3
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| |-
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| | 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
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| |}
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| ==Data==
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| Run#1
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
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| |-
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| | 200||0.000116022
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| |-
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| | 150||0.000192818
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| | 100||0.000139227
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| | 40||5.55556E-06
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| | 16||6.68508E-05
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| | 6.4||0.000039
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| |}
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
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| |-
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| | 0.005||8619.047619
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| | 0.006666667||5186.246418
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| | 0.01||7182.539683
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| |-
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| | 0.025||180000
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| | 0.0625||14958.67769
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| |-
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| | 0.15625||25641.02564
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| |}
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| Run#2
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
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| |-
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| | 200||0.00021989
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| |-
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| | 150||0.000147514
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| |-
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| | 100||0.000144751
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| |-
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| | 40||0.00010884
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| |-
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| | 16||8.12155E-05
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| |-
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| | 6.4||3.8674E-05
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| |}
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| ==Notes== | | ==Notes== |