Entry title
Objective
Learn how to maintain an OpenWetWare Notebook.
Description
- Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
- 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
- Dilution Tables are located below
Concentration Tables
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
| [Old Adenosine Stock Solution] (uM)
|
Volume Adenosine Stock Solution added (uL)
|
Volume Adenosine Phosphate Buffer added (uL)
|
[New Adenosine Stock Solution] (uL)
|
Volume New Adenosine Stock Solution (mL)
|
| 5000 |
200 |
800 |
1000 |
1
|
| 5000 |
150 |
850 |
750 |
1
|
| 5000 |
100 |
900 |
500 |
1
|
| 5000 |
40 |
960 |
200 |
1
|
| 5000 |
16 |
984 |
80 |
1
|
| 5000 |
6.4 |
993.6 |
32 |
1
|
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
| [ADA Stock Solution] (U/mL)
|
Volume ADA Stock Solution Added (mL)
|
Final [ADA] (U/mL)
|
[Adenosine Stock Solution] (mL)
|
Volume Adenosine Stock Solution Added (mL)
|
Final [Adenosine] (uM)
|
Volume 0.05 M Phosphate Buffer Added (mL)
|
Total Volume (mL)
|
| 0.5 |
0.02 |
0.00333 |
1000 |
0.6 |
200 |
2.38 |
3
|
| 0.5 |
0.02 |
0.00333 |
750 |
0.6 |
150 |
2.38 |
3
|
| 0.5 |
0.02 |
0.00333 |
500 |
0.6 |
100 |
2.38 |
3
|
| 0.5 |
0.02 |
0.00333 |
200 |
0.6 |
40 |
2.38 |
3
|
| 0.5 |
0.02 |
0.00333 |
80 |
0.6 |
16 |
2.38 |
3
|
| 0.5 |
0.02 |
0.00333 |
32 |
0.6 |
6.4 |
2.38 |
3
|
Data
Run#1
| Concentration of Adenosine (uM)
|
Average Velocity over 180s (nm/sec)
|
| 200 |
0.000116022
|
| 150 |
0.000192818
|
| 100 |
0.000139227
|
| 40 |
5.55556E-06
|
| 16 |
6.68508E-05
|
| 6.4 |
0.000039
|
| 1/Concentration of Adenosine (uM)
|
1/Velocity (nm/sec)
|
| 0.005 |
8619.047619
|
| 0.006666667 |
5186.246418
|
| 0.01 |
7182.539683
|
| 0.025 |
180000
|
| 0.0625 |
14958.67769
|
| 0.15625 |
25641.02564
|
Run#2
| Concentration of Adenosine (uM)
|
Average Velocity over 180s (nm/sec)
|
| 200 |
0.00021989
|
| 150 |
0.000147514
|
| 100 |
0.000144751
|
| 40 |
0.00010884
|
| 16 |
8.12155E-05
|
| 6.4 |
3.8674E-05
|
| 1/Concentration of Adenosine (uM)
|
1/Velocity (nm/sec)
|
| 0.005 |
4547.738693
|
| 0.006666667 |
6779.026217
|
| 0.01 |
6908.396947
|
| 0.025 |
9187.817259
|
| 0.0625 |
12312.92517
|
| 0.15625 |
25857.14286
|
Notes
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
| 
Biomaterials Design Lab
