User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/13

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==Objective==
==Objective==
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Learn how to maintain an OpenWetWare Notebook.
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Use data collected yesterday to calculate Average values of the Assay and calculate Km
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==Description==
 
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*Calculations using all three assays were performed:
 
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# Velocity by Concentration was graphed for each run
 
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# An average velocity of all three runs was graphed by concentration
 
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# A Lineweaver-Burke Plot was graphed for each run and Km was calculated
 
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# A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated
 
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# Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values
 
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*Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
 
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# An Average velocity of combine data was graphed by concentration
 
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# A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated
 
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# Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values
 
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==Data==
 
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* All Graphs and Tables are for the Combined Data:
 
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200 0.000180385 0.005 5543.691027
 
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150 0.000183794 0.006666667 5440.881764
 
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100 0.000162678 0.01 6147.11077
 
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40 0.000111981 0.025 8930.093256
 
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16 7.95434E-05 0.0625 12571.75005
 
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6.4 3.42451E-05 0.15625 29201.27683
 
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==Notes==
 
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This area is for any observations or conclusions that you would like to note.
 

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Objective

Use data collected yesterday to calculate Average values of the Assay and calculate Km




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