User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/13

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Use data collected yesterday to calculate Average values of the Assay and calculate Km
Use data collected yesterday to calculate Average values of the Assay and calculate Km
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==Description==
 
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*Calculations using all three assays were performed:
 
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# Velocity by Concentration was graphed for each run
 
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# An average velocity of all three runs was graphed by concentration
 
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# A Lineweaver-Burke Plot was graphed for each run and Km was calculated
 
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# A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated
 
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# Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values
 
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*Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
 
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# An Average velocity of combine data was graphed by concentration
 
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# A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated
 
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# Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values
 
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==Data==
 
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* All Graphs and Tables are for the Combined Data:
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Final Concentration Adenosine (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Average velocity over 180 seconds (nm/sec)'''
 
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| align="center" style="background:#f0f0f0;"|'''1/[Adenosine] (1/uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''1/average velocity (sec/nm)'''
 
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|-
 
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| 200||0.000180385||0.005||5543.691027
 
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|-
 
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| 150||0.000183794||0.006666667||5440.881764
 
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|-
 
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| 100||0.000162678||0.01||6147.11077
 
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|-
 
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| 40||0.000111981||0.025||8930.093256
 
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|-
 
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| 16||7.95434E-05||0.0625||12571.75005
 
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|-
 
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| 6.4||3.42451E-05||0.15625||29201.27683
 
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|}
 
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[[Image:Combined_Average_Velocity_over_COncentration.png]]
 
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[[Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png]]
 
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* Km calculations
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Equation'''
 
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| align="center" style="background:#f0f0f0;"|'''Value calculated'''
 
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|-
 
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| (-1)/Km||0.028053516
 
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|-
 
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| Km||35.64615572
 
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|-
 
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| 1/Vmax||4440.372695
 
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|-
 
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| Vmax||0.000225206
 
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|}
 
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==Notes==
 
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* For run #2 concentration 40 uM was removed as an outlier
 
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* For run #1 no values for concentration 150 uM were used
 
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* Ki values for the inhibitors were found in a paper from Planta Medica:
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Flavanoid'''
 
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| align="center" style="background:#f0f0f0;"|'''Ki value)'''
 
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|-
 
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| Myricetin||32 uM
 
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|-
 
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| Kaempferol||32 uM
 
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|-
 
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| Quericetin||26 uM
 
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|}
 

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Use data collected yesterday to calculate Average values of the Assay and calculate Km




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