Entry title
Objective
Use data collected yesterday to calculate Average values of the Assay and calculate Km
Description
 Calculations using all three assays were performed:
 Velocity by Concentration was graphed for each run
 An average velocity of all three runs was graphed by concentration
 A LineweaverBurke Plot was graphed for each run and Km was calculated
 A LineweaverBurke Plot was graphed for the average of the three runs and an average Km was calculated
 Error bars were added to the average LineweaverBurke Plot by calculating the standard deviation of the three runs 1/velocity values
 Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
 An Average velocity of combine data was graphed by concentration
 A LineweaverBurke Plot was graphed for the average of the combined data and an average Km was calculated
 Error bars were added to the combined data average LineweaverBurke plot by calculating the standard deviation of the combined data 1/velocity values
Data
 All Graphs and Tables are for the Combined Data:
Final Concentration Adenosine (uM)
 Average velocity over 180 seconds (nm/sec)
 1/[Adenosine] (1/uM)
 1/average velocity (sec/nm)

200  0.000180385  0.005  5543.691027

150  0.000183794  0.006666667  5440.881764

100  0.000162678  0.01  6147.11077

40  0.000111981  0.025  8930.093256

16  7.95434E05  0.0625  12571.75005

6.4  3.42451E05  0.15625  29201.27683

Equation
 Value calculated

(1)/Km  0.028053516

Km  35.64615572

1/Vmax  4440.372695

Vmax  0.000225206

Notes
 For run #2 concentration 40 uM was removed as an outlier
 For run #1 no values for concentration 150 uM were used
 Ki values for the inhibitors were found in a paper from Planta Medica:
Flavanoid
 Ki value)

Myricetin  32 uM

Kaempferol  32 uM

Quericetin  26 uM

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