User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/13

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==Objective==
==Objective==
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Learn how to maintain an OpenWetWare Notebook.
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Use data collected yesterday to calculate Average values of the Assay and calculate Km
==Description==
==Description==
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==Data==
==Data==
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* Add data and results here...
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* All Graphs and Tables are for the Combined Data:
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Final Concentration Adenosine (uM)'''
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| align="center" style="background:#f0f0f0;"|'''Average velocity over 180 seconds (nm/sec)'''
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| align="center" style="background:#f0f0f0;"|'''1/[Adenosine] (1/uM)'''
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| align="center" style="background:#f0f0f0;"|'''1/average velocity (sec/nm)'''
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|-
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| 200||0.000180385||0.005||5543.691027
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|-
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| 150||0.000183794||0.006666667||5440.881764
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|-
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| 100||0.000162678||0.01||6147.11077
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|-
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| 40||0.000111981||0.025||8930.093256
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|-
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| 16||7.95434E-05||0.0625||12571.75005
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|-
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| 6.4||3.42451E-05||0.15625||29201.27683
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|}
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[[Image:Combined_Average_Velocity_over_COncentration.png]]
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[[Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png]]
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* Km calculations
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Equation'''
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| align="center" style="background:#f0f0f0;"|'''Value calculated'''
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|-
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| (-1)/Km||0.028053516
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|-
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| Km||35.64615572
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|-
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| 1/Vmax||4440.372695
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|-
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| Vmax||0.000225206
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|}
==Notes==
==Notes==
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This area is for any observations or conclusions that you would like to note.
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* For run #2 concentration 40 uM was removed as an outlier
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* For run #1 no values for concentration 150 uM were used
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* Ki values for the inhibitors were found in a paper from Planta Medica:
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Flavanoid'''
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| align="center" style="background:#f0f0f0;"|'''Ki value)'''
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|-
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| Myricetin||32 uM
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|-
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| Kaempferol||32 uM
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|-
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| Quericetin||26 uM
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|}

Revision as of 14:20, 15 February 2013

Biomaterials Design Lab Main project page
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Entry title

Objective

Use data collected yesterday to calculate Average values of the Assay and calculate Km

Description

  • Calculations using all three assays were performed:
  1. Velocity by Concentration was graphed for each run
  2. An average velocity of all three runs was graphed by concentration
  3. A Lineweaver-Burke Plot was graphed for each run and Km was calculated
  4. A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated
  5. Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values
  • Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
  1. An Average velocity of combine data was graphed by concentration
  2. A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated
  3. Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values

Data

  • All Graphs and Tables are for the Combined Data:
Final Concentration Adenosine (uM) Average velocity over 180 seconds (nm/sec) 1/[Adenosine] (1/uM) 1/average velocity (sec/nm)
2000.0001803850.0055543.691027
1500.0001837940.0066666675440.881764
1000.0001626780.016147.11077
400.0001119810.0258930.093256
167.95434E-050.062512571.75005
6.43.42451E-050.1562529201.27683

Image:Combined_Average_Velocity_over_COncentration.png

Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png


  • Km calculations
Equation Value calculated
(-1)/Km0.028053516
Km35.64615572
1/Vmax4440.372695
Vmax0.000225206

Notes

  • For run #2 concentration 40 uM was removed as an outlier
  • For run #1 no values for concentration 150 uM were used
  • Ki values for the inhibitors were found in a paper from Planta Medica:
Flavanoid Ki value)
Myricetin32 uM
Kaempferol32 uM
Quericetin26 uM


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