User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/13 - Revision history

Mary Mendoza: /* Notes */

Mary Mendoza — Wed, 08 May 2013 21:51:50 GMT

Notes

←Older revision		Revision as of 21:51, 8 May 2013
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-	~~==Notes==~~
-	* For run #2 concentration 40 uM was removed as an outlier
-	* For run #1 no values for concentration 150 uM were used
-	* Ki values for the inhibitors were found in a paper from Planta Medica:
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Flavanoid'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Ki value)'''~~
-	\|-
-	~~\| Myricetin\|\|32 uM~~
-	\|-
-	~~\| Kaempferol\|\|32 uM~~
-	\|-
-	~~\| Quericetin\|\|26 uM~~
-	\|}

Mary Mendoza — Wed, 08 May 2013 21:51:30 GMT

Data

←Older revision		Revision as of 21:51, 8 May 2013
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-	~~==Data==~~
-	* All Graphs and Tables are for the Combined Data:

-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Final Concentration Adenosine (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Average velocity over 180 seconds (nm/sec)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/[Adenosine] (1/uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/average velocity (sec/nm)'''~~
-	\|-
-	~~\| 200\|\|0.000180385\|\|0.005\|\|5543.691027~~
-	\|-
-	~~\| 150\|\|0.000183794\|\|0.006666667\|\|5440.881764~~
-	\|-
-	~~\| 100\|\|0.000162678\|\|0.01\|\|6147.11077~~
-	\|-
-	~~\| 40\|\|0.000111981\|\|0.025\|\|8930.093256~~
-	\|-
-	~~\| 16\|\|7.95434E-05\|\|0.0625\|\|12571.75005~~
-	\|-
-	~~\| 6.4\|\|3.42451E-05\|\|0.15625\|\|29201.27683~~
-	\|}
-
-	~~[[Image:Combined_Average_Velocity_over_COncentration.png]]~~
-
-	~~[[Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png]]~~
-
-
-	* Km calculations
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Equation'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Value calculated'''~~
-	\|-
-	~~\| (-1)/Km\|\|0.028053516~~
-	\|-
-	~~\| Km\|\|35.64615572~~
-	\|-
-	~~\| 1/Vmax\|\|4440.372695~~
-	\|-
-	~~\| Vmax\|\|0.000225206~~
-	\|}

	==Notes==		==Notes==

Mary Mendoza — Wed, 08 May 2013 21:51:17 GMT

Description

←Older revision		Revision as of 21:51, 8 May 2013
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	Use data collected yesterday to calculate Average values of the Assay and calculate Km		Use data collected yesterday to calculate Average values of the Assay and calculate Km

-	~~==Description==~~
-	*Calculations using all three assays were performed:
-	~~# Velocity by Concentration was graphed for each run~~
-	~~# An average velocity of all three runs was graphed by concentration~~
-	~~# A Lineweaver-Burke Plot was graphed for each run and Km was calculated~~
-	~~# A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated~~
-	~~# Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values~~

-	*Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
-	~~# An Average velocity of combine data was graphed by concentration~~
-	~~# A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated~~
-	~~# Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values~~

	==Data==		==Data==

Catherine Koenigsknecht — Fri, 15 Feb 2013 18:20:24 GMT

Notes

←Older revision		Revision as of 18:20, 15 February 2013
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	==Notes==		==Notes==
-	* For run #2 concentration ~~6.4~~ uM was removed as an outlier	+	* For run #2 concentration 40 uM was removed as an outlier
	* For run #1 no values for concentration 150 uM were used		* For run #1 no values for concentration 150 uM were used
		+	* Ki values for the inhibitors were found in a paper from Planta Medica:
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Flavanoid'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Ki value)'''
		+	\|-
		+	\| Myricetin\|\|32 uM
		+	\|-
		+	\| Kaempferol\|\|32 uM
		+	\|-
		+	\| Quericetin\|\|26 uM
		+	\|}

Catherine Koenigsknecht — Fri, 15 Feb 2013 18:15:49 GMT

Objective

←Older revision		Revision as of 18:15, 15 February 2013
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	==Objective==		==Objective==
-	~~Learn how~~ to ~~maintain an OpenWetWare Notebook.~~	+	Use data collected yesterday to calculate Average values of the Assay and calculate Km

	==Description==		==Description==

Catherine Koenigsknecht — Fri, 15 Feb 2013 18:13:39 GMT

Notes

←Older revision		Revision as of 18:13, 15 February 2013
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	==Notes==		==Notes==
-	~~This area is for any observations or conclusions that you would like to note~~.	+	* For run #2 concentration 6.4 uM was removed as an outlier
		+	* For run #1 no values for concentration 150 uM were used

Catherine Koenigsknecht — Fri, 15 Feb 2013 18:12:16 GMT

Data

←Older revision		Revision as of 18:12, 15 February 2013
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	[[Image:Combined_Average_Velocity_over_COncentration.png]]		[[Image:Combined_Average_Velocity_over_COncentration.png]]
		+
		+	[[Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png]]

Catherine Koenigsknecht — Fri, 15 Feb 2013 18:11:28 GMT

Data

←Older revision		Revision as of 18:11, 15 February 2013
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	\|}		\|}

-	[[~~http://openwetware.org/wiki/~~Image:Combined_Average_Velocity_over_COncentration.png]]	+	[[Image:Combined_Average_Velocity_over_COncentration.png]]

Catherine Koenigsknecht — Fri, 15 Feb 2013 18:10:03 GMT

Data

←Older revision		Revision as of 18:10, 15 February 2013
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	\| 6.4\|\|3.42451E-05\|\|0.15625\|\|29201.27683		\| 6.4\|\|3.42451E-05\|\|0.15625\|\|29201.27683
	\|}		\|}
		+
		+	[[http://openwetware.org/wiki/Image:Combined_Average_Velocity_over_COncentration.png]]
		+

	* Km calculations		* Km calculations

Catherine Koenigsknecht — Fri, 15 Feb 2013 17:58:19 GMT

Data

←Older revision		Revision as of 17:58, 15 February 2013
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	\|-		\|-
	\| 6.4\|\|3.42451E-05\|\|0.15625\|\|29201.27683		\| 6.4\|\|3.42451E-05\|\|0.15625\|\|29201.27683
		+	\|}
		+
		+	* Km calculations
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Equation'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Value calculated'''
		+	\|-
		+	\| (-1)/Km\|\|0.028053516
		+	\|-
		+	\| Km\|\|35.64615572
		+	\|-
		+	\| 1/Vmax\|\|4440.372695
		+	\|-
		+	\| Vmax\|\|0.000225206
	\|}		\|}