User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/13

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Entry title

Objective

Use data collected yesterday to calculate Average values of the Assay and calculate Km


Data

  • All Graphs and Tables are for the Combined Data:
Final Concentration Adenosine (uM) Average velocity over 180 seconds (nm/sec) 1/[Adenosine] (1/uM) 1/average velocity (sec/nm)
2000.0001803850.0055543.691027
1500.0001837940.0066666675440.881764
1000.0001626780.016147.11077
400.0001119810.0258930.093256
167.95434E-050.062512571.75005
6.43.42451E-050.1562529201.27683

Image:Combined_Average_Velocity_over_COncentration.png

Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png


  • Km calculations
Equation Value calculated
(-1)/Km0.028053516
Km35.64615572
1/Vmax4440.372695
Vmax0.000225206

Notes

  • For run #2 concentration 40 uM was removed as an outlier
  • For run #1 no values for concentration 150 uM were used
  • Ki values for the inhibitors were found in a paper from Planta Medica:
Flavanoid Ki value)
Myricetin32 uM
Kaempferol32 uM
Quericetin26 uM


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