User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/27

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Objective)
(Entry title)
(2 intermediate revisions not shown.)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
 
-
 
Line 15: Line 13:
==Description==
==Description==
-
#  
+
*Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
 +
**The following concentrations were used.
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration [Ado] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Velocity (nm/sec)'''
 +
| align="center" style="background:#f0f0f0;"|'''1/[Ado] (1/uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''1/velocity (sec/nm)'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
| align="center" style="background:#f0f0f0;"|'''(-1)/Km'''
 +
| align="center" style="background:#f0f0f0;"|'''-0.022385428'''
 +
|-
 +
| 10.52||3.70166E-05||0.095057034||27014.92537||||Km||44.67191792
 +
|-
 +
| 12.34||5.13812E-05||0.081037277||19462.36559||||1/Vmax||2662.131337
 +
|-
 +
| 14.93||6.0221E-05||0.066979236||16605.50459||||Vmax||0.000375639
 +
|-
 +
| 18.87||7.45856E-05||0.052994171||13407.40741||||||
 +
|-
 +
| 25.64||8.67403E-05||0.03900156||11528.66242||||||
 +
|-
 +
| 40||9.94475E-05||0.025||10055.55556||||||
 +
|-
 +
|
 +
|}
 +
 
 +
*10mM Quercetin stock was prepared.
 +
*Cuvettes with the following solutions were prepared and analyzed.
 +
**Today, we only had time to complete the first two.
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Inhibitor Stock solution added'''
 +
| align="center" style="background:#f0f0f0;"|'''[Inhibitor Stock Solution] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''[Final Inhibitor] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
 +
|-
 +
| 0.5||0.02||0.00333||52.63157895||0.6||10.52631579||1.78||0.6||65||13||3
 +
|-
 +
| 0.5||0.02||0.00333||61.72839506||0.6||12.34567901||1.78||0.6||65||13||3
 +
|-
 +
| 0.5||0.02||0.00333||74.62686567||0.6||14.92537313||1.78||0.6||65||13||3
 +
|-
 +
| 0.5||0.02||0.00333||94.33962264||0.6||18.86792453||1.78||0.6||65||13||3
 +
|-
 +
| 0.5||0.02||0.00333||128.2051282||0.6||25.64102564||1.78||0.6||65||13||3
 +
|-
 +
| 0.5||0.02||0.00333||200||0.6||40||1.78||0.6||65||13||3
 +
|-
 +
|
 +
|}
==Data==
==Data==
-
* Add data and results here...
+
*The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.  
 +
[[Image:3_5data.JPG]]
 +
 
 +
*Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.
==Notes==
==Notes==

Revision as of 21:54, 1 April 2013

Biomaterials Design Lab Main project page
Previous entry      Next entry


Objective

  • Finish Kinetic Data and calculate average
  • Begin UV runs with calculated inhibitor

Description

  • Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
    • The following concentrations were used.
Concentration [Ado] (uM) Velocity (nm/sec) 1/[Ado] (1/uM) 1/velocity (sec/nm) ' (-1)/Km -0.022385428
10.523.70166E-050.09505703427014.92537Km44.67191792
12.345.13812E-050.08103727719462.365591/Vmax2662.131337
14.936.0221E-050.06697923616605.50459Vmax0.000375639
18.877.45856E-050.05299417113407.40741
25.648.67403E-050.0390015611528.66242
409.94475E-050.02510055.55556
  • 10mM Quercetin stock was prepared.
  • Cuvettes with the following solutions were prepared and analyzed.
    • Today, we only had time to complete the first two.
[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Volume Inhibitor Stock solution added [Inhibitor Stock Solution] (uM) [Final Inhibitor] (uM) Total Volume (mL)
0.50.020.0033352.631578950.610.526315791.780.665133
0.50.020.0033361.728395060.612.345679011.780.665133
0.50.020.0033374.626865670.614.925373131.780.665133
0.50.020.0033394.339622640.618.867924531.780.665133
0.50.020.00333128.20512820.625.641025641.780.665133
0.50.020.003332000.6401.780.665133

Data

  • The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.

Image:3_5data.JPG

  • Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Personal tools