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| |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> |
| |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} |
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| ==Description==
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| *Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
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| **The following concentrations were used.
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''Concentration [Ado] (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''Velocity (nm/sec)'''
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| | align="center" style="background:#f0f0f0;"|'''1/[Ado] (1/uM)'''
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| | align="center" style="background:#f0f0f0;"|'''1/velocity (sec/nm)'''
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| | align="center" style="background:#f0f0f0;"|''''''
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| | align="center" style="background:#f0f0f0;"|'''(-1)/Km'''
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| | align="center" style="background:#f0f0f0;"|'''-0.022385428'''
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| |-
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| | 10.52||3.70166E-05||0.095057034||27014.92537||||Km||44.67191792
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| |-
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| | 12.34||5.13812E-05||0.081037277||19462.36559||||1/Vmax||2662.131337
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| |-
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| | 14.93||6.0221E-05||0.066979236||16605.50459||||Vmax||0.000375639
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| |-
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| | 18.87||7.45856E-05||0.052994171||13407.40741||||||
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| |-
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| | 25.64||8.67403E-05||0.03900156||11528.66242||||||
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| |-
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| | 40||9.94475E-05||0.025||10055.55556||||||
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| |-
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| |}
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| *10mM Quercetin stock was prepared.
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| *Cuvettes with the following solutions were prepared and analyzed.
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| **Today, we only had time to complete the first two.
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
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| | align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
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| | align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
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| | align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
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| | align="center" style="background:#f0f0f0;"|'''Volume Inhibitor Stock solution added'''
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| | align="center" style="background:#f0f0f0;"|'''[Inhibitor Stock Solution] (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''[Final Inhibitor] (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
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| |-
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| | 0.5||0.02||0.00333||52.63157895||0.6||10.52631579||1.78||0.6||65||13||3
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| |-
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| | 0.5||0.02||0.00333||61.72839506||0.6||12.34567901||1.78||0.6||65||13||3
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| |-
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| | 0.5||0.02||0.00333||74.62686567||0.6||14.92537313||1.78||0.6||65||13||3
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| |-
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| | 0.5||0.02||0.00333||94.33962264||0.6||18.86792453||1.78||0.6||65||13||3
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| |-
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| | 0.5||0.02||0.00333||128.2051282||0.6||25.64102564||1.78||0.6||65||13||3
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| |-
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| | 0.5||0.02||0.00333||200||0.6||40||1.78||0.6||65||13||3
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| |-
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| |}
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| ==Data==
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| *The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.
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| [[Image:3_5data.JPG]]
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| *Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.
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| ==Notes== | | ==Notes== |