User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/03/05

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Entry title

Objective

  1. Run UV-Vis with inhibitor concentration 13 uM over all calculated Adenosine solution concentrations
  2. Run UV-Vis with inhibitor concentration 26 uM over all calculated Adenosine solution concentrations
  3. Plot Lineweaver-Burke Plots of Trials and compare trendlines of different concentrations of the inhibitor

Description

  • Adenosine Solutions were prepared with the previous method:

Image:Picture_14.png

  • Inhibitor Solution preparation:


  • Final Mixture in the Cuvette:

Image:Picture_4.png

Data

  • UV-Vis Data 13uM inhibitor:

Image:13uMvelocity.png

  • Graph of Adenosine with inhibitor vs Adenosine only:

Image:Picture_12.png


  • UV-Vis Data 26 uM inhibitor:

Image:26uMvelocity.png

  • Graph of Adenosine with inhibitor vs Adenosine only:

Image:Picture_13.png

Notes

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