User:Chris D Hirst/Protocols: Difference between revisions
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==Protocol 3== | |||
Revision as of 02:36, 6 August 2008
Protocols
Here will be placed any finished or currently worked on protocols until tehy are deemed of a high enough standard to be put on the Imperial iGEM 08 wetware wiki.
Cell Count v Optical Density Curve Calibration
Aim
To produce a calibration curve to aid in the normalising of fluorescence values to allow proper characterisation of Promoters and RBSs for B.subitlis. This protocol must give results that are as accurate as possible over a considerable range of Optical Densities.
Equipment
Spectrophotometer Cuvettes P20, P200 and P1000 Gilsons 50mL flask (an additional one for each repeat) Timer
Reagents
LB Medium LB Agar plates (x )
Protocol
1. Grow up a culture of B.subtilis overnight 2. Pipette 500μL
