User:Chris D Hirst/Protocols

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Protocols

Here will be placed any finished or currently worked on protocols until tehy are deemed of a high enough standard to be put on the Imperial iGEM 08 wetware wiki.

Cell Count v Optical Density Curve Calibration

Aim

To produce a calibration curve to aid in the normalising of fluorescence values to allow proper characterisation of Promoters and RBSs for B.subitlis. This protocol must give results that are as accurate as possible over a considerable range of Optical Densities.

Equipment


Reagents


Protocol


Protocol #2