User:Corey Bear/Notebook/09 July 2014 Identifying and Studying Bacteria

Intent: The intent of this experiment was to understand the characteristics of bacteria to observe antibiotic resistance, and to properly identify DNA sequences presence from PCR in later processing.

Overview: During this lab, the serial dilution results were observed. Two agar plates were chosen from both the nutrient positive and tetracycline plates, and extracted utilizing sterilized tools to conduct a Gram Stain Procedure. Additionally, each of the four agar plates that were observed also had content extracted to three 16s DNA sequencing tubes for the process of performing a PCR.

Observations: This experiment is controlled under optimal conditions suitable for normal bacteria. Because of this, the probability of archaea presence in the samples is unlikely, due to the extreme conditions in which they thrive. Additional growth from the current bacteria has as smell of rotten food. Continued growth and bacteria proliferation is anticipated to continue due to environmental changes and ample amounts of nutrients. This continued growth is anticipated until either the environment does not suit the organisms’ fitness or nutrient depletion. The agar plates which had tetracycline showed signs of fewer colonies. Under observation, the antibiotic plates had large presence of orange growth in the colonies. This indicated that bacteria, in the presence of antibiotics, are not as prevalent, and therefore temporarily affected by the antibiotics. The overall effect present with tetracycline hinders bacterial growth, but has limited affect on fungi growth. From observation, the orange bacteria present in the agars has either produced a resistance to the antibiotic or is not a bacteria. “Tetracycline works by interfering with the ability of bacteria to produce proteins that are essential to them. Without these proteins the bacteria cannot grow, multiply and increase in numbers. Tetracycline therefore stops the spread of the infection and the remaining bacteria are killed by the immune system or eventually die.” (Netdoctor) Because of this, tetracycline treats bacteria infections of the urinary tract, Syphillis, Gonorrhoea, and other types of bacterial conditions.

During observations under a compound microscope, the following information were observed, cited in (Table 2).

Table 1: https://drive.google.com/file/d/0B0_IEyKChqDxVEdBeFJQWFhlbWc/edit?usp=sharing

Table 2: https://drive.google.com/file/d/0B0_IEyKChqDxQ1JZMF92WjdFa0U/edit?usp=sharing

Reference (Netdoctor) Tetracycline tablets: How does it work? Retrieved from http://www.netdoctor.co.uk/sex-and-relationships/medicines/tetracycline-tablets.html.