User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/20: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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## concentration = 3.73 mM
## concentration = 3.73 mM


<u>Instructions for making sample for ocean optics</u>
# pH 5
# pH 5
## Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
## Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
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### 167 μL Lysozyme
### 167 μL Lysozyme
### 2580 μL Water
### 2580 μL Water


{|border="1" cellpadding="2"
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| 180 || 0.67341 || 535.5
| 180 || 0.67341 || 535.5
|}
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[[Image:MaximumAbsorbance092016.png|800px|]]
[[Image:MaximumWavelength092016.png|800px|]]
[[Image:092016 Abs v Wave Lys OO pH5.png|800px|]]


==Notes==
==Notes==

Latest revision as of 02:01, 27 September 2017

Project name Main project page
Previous entry      Next entry

September 20, 2016 : Ocean Optics II

Objective

  1. Run Ocean optics on sample prepped today.

Description

Ocean Optics

  1. Sample was prepped according to Lysozyme prep seen in data
  2. Sample was placed into Ocean Optics Machine
  3. Machine was run for 3 hours collecting data every 2 minutes

Data

Stock solutions

  1. Lysozyme
    1. 32.24 mg of lysozyme with water in 10.0 mL volumetric flask
    2. MW = 14307 g/mol
    3. concentration = 225 μM
  2. AuCl3
    1. 28.27 mg of AuCl3 with water in 25.0 mL volumetric flask
    2. MW = 303.3 g/mol
    3. concentration = 3.73 mM
  1. pH 5
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 223 μL AuCl3
      2. 30 μL 1M HCl
      3. 167 μL Lysozyme
      4. 2580 μL Water


Summary Data at 30 minute increments
Time(minutes) Maximum Absorbance Maximum Wavelength(nm)
0 0.13438 428
30 0.14248 428
60 0.28637 538.98
90 0.45071 535.5
120 0.55944 538.98
150 0.63036 534.45
180 0.67341 535.5


Notes


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