User:Dadrummond
From OpenWetWare
(Difference between revisions)
(body) |
(→To do) |
||
| Line 6: | Line 6: | ||
**Tests whether results depend on the tag or not | **Tests whether results depend on the tag or not | ||
*Complement CDC19 deletion using acceptor-bracketed allele | *Complement CDC19 deletion using acceptor-bracketed allele | ||
| + | **Tests whether sandwiched proteins are functional | ||
*Optimize soluble/insoluble extraction conditions | *Optimize soluble/insoluble extraction conditions | ||
| + | **Glass bead lysis | ||
| + | **Chemical lysis | ||
==Templates== | ==Templates== | ||
Revision as of 22:54, 8 July 2007
This is Allan's page. Drummond
To do
Controls.
- HXK2 using native antibody
- Tests whether results depend on the tag or not
- Complement CDC19 deletion using acceptor-bracketed allele
- Tests whether sandwiched proteins are functional
- Optimize soluble/insoluble extraction conditions
- Glass bead lysis
- Chemical lysis
Templates
Protocols under construction
Detection of soluble and insoluble proteins: Drummond:Solubility Buffers for lysis: Drummond:Lysis Protein slot-blot: Drummond:Protein Slot Blot Protocol
Test page for CSS columns: Drummond:Columns


