User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2013/10/01
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ObjectiveTo monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection. DescriptionThree sets of measurements will be performed today.
Here is the UV-Vis absorption spectrum for HRP, Luminol and H2O2 obtained. Note that the absorption of HRP is greater than 1 which is why we made a new solution diluted by a factor of 1/20 with the buffer solution.
DataStock Solution
ResultsThere were some complications with the spectra as none of them appeared to be working. We used 1/20 dilutions of the HRP, Luminol and H2O2 and it still did not work possibly because the hydrogen peroxide to luminol ratio was too high. We used the data from Trisha's group for our analysis and the results show a decrease of HRP |